South San FranciscoFull TimeEngineering
Remotely
qpcrddpcrtransfectionchocell line developmentselectionclone screeningtransposase
Job Description
📋 Description
- Develop CHO cell lines for GMP applications in South San Francisco, CA.
- Bench-focused role owning projects end to end with process & analytical partners.
- Execute cell line development workflows: transfection, selection, clone screening, scale-up.
- Characterize lines via PCR/qPCR/ddPCR and sequencing-based methods.
- Generate monoclonal lines and maintain cell banks with proper records.
- Design fed-batch and parallel culture studies to evaluate productivity and stability.
🎯 Requirements
- Bachelor’s degree (5+ yrs) or Master’s (2+ yrs) in biologics or related field.
- Hands-on aseptic mammalian cell culture with CHO focus.
- Proficiency in DNA/RNA extraction and PCR-based analyses (qPCR/ddPCR).
- Experience creating stable CHO lines and using transposase/recombinase systems.
- Cryopreservation of CHO cells and cell bank management.
- Experience with cloning, screening in 96-well/24-deep-well plates, and data interpretation.
🎁 Benefits
- AbbVie-wide benefits and compensation program.
- Opportunity to work on diverse platform/non-platform projects.
- Equal opportunity employer.